依照老師上課PPT第37頁的流程，自己跑一遍
hexosaminidase A
輸入hexosaminidase A
Select gene
會到下列的網址:
接著到視窗右邊的Find related data選nucleotide，再選RefSeqGene
接著再到視窗右邊的Find related data選nucleotide，下列選項會有mRNA
輸入hexosaminidase A AND human[orgn]
及會到下列的網址:
之後至視窗右邊的Find related data選OMIM
其他的連結可以點進去搜尋看看
NCBI還有提供BLAST的功能:
依照老師的PPT 43~50頁的操作方式自我練習一次
我選我做的dengue virus nonstructural protein 1 定序的結果來進行blast
給予的Enter Query Sequence:
>RT-PCR Product
GCTTGGAAAAAGAACTGAAGTGTGGCAGTGGGATTTTCATCACAGACAACGTGCACACATGGACAGAACAATACAAGTTCCAACCAGAATCCCCTTCAAAACTAGCTTCAGCTATCCAGAAAGCTCATGAAGAGGGCATTTGTGGAATCCGCTCAGTAACAAGACTGGAAAATCTGATGTGGAAACAAATAACACCAGAATTGAATCACATTCTATCAGAAAATGAGGTGAAGTTGACTATTATGACAGGAGACATCAAAGGAATCATGCAGGCAGGAAAACGATCTCTGCGGCCCCAGCCCACTGAGCTGAAGTATTCATGGAAAACATGGGGCAAAGCGAAAATGCTCTCTACAGAGTCTCATAACCAGACCTTTCTCATTGATGGCCCCGAAACAGCAGAATGCCCCAACACAAACAGAGCTTGGAATTCGCTGGAAGTTGAAGACTATGGCTTTGGAGTATTCACCACCAATATATGGCTAAAGTTGAGAGAAAAGCAGGATGTATTCTGCGACTCAAAACTCATGTCAGCGGCCATAAAAGACAACAGAGCCGTCCATGCCGATATGGGTTATTGGATAGAAAGTGCACTCAATGACACATGGAAGATAGAGAAAGCCTCTTTCATCGAAGTTAAAAGCTGCCACTGGCCAAAGTCACACACCCTCTGGAGTAATGAAGTGCTAGAAAGTGAGATGATAATTCCAAAGAATTTCGCTGGACCAGTGTCACAACACAACTACAGACCAGGCTACCATACACAAACAGCAGGACCATGGCATCTAGGTAAGCTTGAGATGGACTTTGATTTCTGCGAAGGAACCACAGTGGTGGTGACTGAGGACTGTGGAAATAGAGGACCCTCTTTAAGAACAACTACTGCCTCTGGAAAACTCATAACAGAATGGTGCTGCCGATCTCGCACATTACCACCGCTAAGATACAGAGGTGAGGACGGATGCTGGTACGGGATGGAAATCAGACCATTGAAAGAGAAAGAAGAGAATTTGGTCAACTCCTTGGTCACAGCCCTGACTCGAGCACCACC
Enter Subject Sequence:
從 Viral Bioinformatics Resource Center所得到的Sequence
BLAST的結果如下:
BLASTN 2.2.24+
Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and
Webb Miller (2000), "A greedy algorithm for aligning DNA
sequences", J Comput Biol 2000; 7(1-2):203-14.
RID: BXYEDGMT111
Query= RT-PCR Product
Length=1051
Score E
Sequences producing significant alignments: (Bits) Value
lcl|50941 NGC strain 1868 0.0
ALIGNMENTS
>lcl|50941 NGC strain
Length=1056
Score = 1868 bits (1011), Expect = 0.0
Identities = 1021/1026 (99%), Gaps = 0/1026 (0%)
Strand=Plus/Plus
Query 9 AAAGAACTGAAGTGTGGCAGTGGGATTTTCATCACAGACAACGTGCACACATGGACAGAA 68
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 31 AAAGAACTGAAGTGTGGCAGTGGGATTTTCATCACAGACAACGTGCACACATGGACAGAA 90
Query 69 CAATACAAGTTCCAACCAGAATCCCCTTCAAAACTAGCTTCAGCTATCCAGAAAGCTCAT 128
|||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
Sbjct 91 CAATACAAGTTCCAACCAGAATCCCCTTCAAAGCTAGCTTCAGCTATCCAGAAAGCTCAT 150
Query 129 GAAGAGGGCATTTGTGGAATCCGCTCAGTAACAAGACTGGAAAATCTGATGTGGAAACAA 188
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 151 GAAGAGGGCATTTGTGGAATCCGCTCAGTAACAAGACTGGAAAATCTGATGTGGAAACAA 210
Query 189 ATAACACCAGAATTGAATCACATTCTATCAGAAAATGAGGTGAAGTTGACTATTATGACA 248
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 211 ATAACACCAGAATTGAATCACATTCTATCAGAAAATGAGGTGAAGTTGACTATTATGACA 270
Query 249 GGAGACATCAAAGGAATCATGCAGGCAGGAAAACGATCTCTGCGGCCCCAGCCCACTGAG 308
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
Sbjct 271 GGAGACATCAAAGGAATCATGCAGGCAGGAAAACGATCTCTGCAGCCCCAGCCCACTGAG 330
Query 309 CTGAAGTATTCATGGAAAACATGGGGCAAAGCGAAAATGCTCTCTACAGAGTCTCATAAC 368
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 331 CTGAAGTATTCATGGAAAACATGGGGCAAAGCGAAAATGCTCTCTACAGAGTCTCATAAC 390
Query 369 CAGACCTTTCTCATTGATGGCCCCGAAACAGCAGAATGCCCCAACACAAACAGAGCTTGG 428
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 391 CAGACCTTTCTCATTGATGGCCCCGAAACAGCAGAATGCCCCAACACAAACAGAGCTTGG 450
Query 429 AATTCGCTGGAAGTTGAAGACTATGGCTTTGGAGTATTCACCACCAATATATGGCTAAAG 488
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 451 AATTCGCTGGAAGTTGAAGACTATGGCTTTGGAGTATTCACCACCAATATATGGCTAAAG 510
Query 489 TTGAGAGAAAAGCAGGATGTATTCTGCGACTCAAAACTCATGTCAGCGGCCATAAAAGAC 548
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 511 TTGAGAGAAAAGCAGGATGTATTCTGCGACTCAAAACTCATGTCAGCGGCCATAAAAGAC 570
Query 549 AACAGAGCCGTCCATGCCGATATGGGTTATTGGATAGAAAGTGCACTCAATGACACATGG 608
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 571 AACAGAGCCGTCCATGCCGATATGGGTTATTGGATAGAAAGTGCACTCAATGACACATGG 630
Query 609 AAGATAGAGAAAGCCTCTTTCATCGAAGTTAAAAGCTGCCACTGGCCAAAGTCACACACC 668
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 631 AAGATAGAGAAAGCCTCTTTCATCGAAGTTAAAAGCTGCCACTGGCCAAAGTCACACACC 690
Query 669 CTCTGGAGTAATGAAGTGCTAGAAAGTGAGATGATAATTCCAAAGAATTTCGCTGGACCA 728
||||||||||||| |||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 691 CTCTGGAGTAATGGAGTGTTAGAAAGTGAGATGATAATTCCAAAGAATTTCGCTGGACCA 750
Query 729 GTGTCACAACACAACTACAGACCAGGCTACCATACACAAACAGCAGGACCATGGCATCTA 788
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 751 GTGTCACAACACAACTACAGACCAGGCTACCATACACAAACAGCAGGACCATGGCATCTA 810
Query 789 GGTAAGCTTGAGATGGACTTTGATTTCTGCGAAGGAACCACAGTGGTGGTGACTGAGGAC 848
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 811 GGTAAGCTTGAGATGGACTTTGATTTCTGCGAAGGAACCACAGTGGTGGTGACTGAGGAC 870
Query 849 TGTGGAAATAGAGGACCCTCTTTAAGAACAACTACTGCCTCTGGAAAACTCATAACAGAA 908
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 871 TGTGGAAATAGAGGACCCTCTTTAAGAACAACTACTGCCTCTGGAAAACTCATAACAGAA 930
Query 909 TGGTGCTGCCGATCTCGCACATTACCACCGCTAAGATACAGAGGTGAGGACGGATGCTGG 968
||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
Sbjct 931 TGGTGCTGCCGATCTTGCACATTACCACCGCTAAGATACAGAGGTGAGGACGGATGCTGG 990
Query 969 TACGGGATGGAAATCAGACCATTGAAAGAGAAAGAAGAGAATTTGGTCAACTCCTTGGTC 1028
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 991 TACGGGATGGAAATCAGACCATTGAAAGAGAAAGAAGAGAATTTGGTCAACTCCTTGGTC 1050
Query 1029 ACAGCC 1034
||||||
Sbjct 1051 ACAGCC 1056
